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Customer Notification
Information for Customers Requiring DNA Analysis
The Forensic DNA department of Cuyahoga County Regional Forensic Science Laboratory performs autosomal and Y-STR DNA analysis. DNA STR analysis involves extraction, quantification, amplification, detection, data analysis and statistical interpretation. The following methods are used for DNA STR analysis by the DNA Department:
Extraction:
- Organic extraction and Maxwell 16 Extraction using DNA IQ Casework Pro kit
Quantification:
- Real Time PCR using Quantifiler Duo DNA Quantification kit
- Instrument: AB 7500 SDS Real Time PCR Instrument
Amplification:
- Autosomal STR Amplification kit: PowerPlex Fusion
- Y- STR Amplification kit: PowerPlex Y23
- Instrument: GeneAmp PCR System 9700
Detection:
- AB 3500 Genetic Analyzers
Data Analysis:
Statistical Interpretation:
- Autosomal STR: FBI Popstats, TrueAllele Technology
- Y-STR: U.S. Y-STR database